Thread: MiSeq
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Old 02-21-2011, 09:20 AM   #45
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Originally Posted by james hadfield View Post
Vinz: The MiSeq completes a PE100 run in 1 day compared to 8 on HiSeq and 12 on GAIIx. If you look at intensities and then error profiles for runs they drop off at the end. Much of this is due to the time the reagents sit on the instrument and Illumina only support ten days.
MiSeq runs fast, very fast. I am hoping that this has an impact on quality and would not be surprised to see users publishing 200, 300 and even higher read lengths. Lets wait and see when people get hold of them.
I would guess that the decay of phasing and overall efficiencies would be a bigger limitation than reagent stability. Even at >99% efficiency, for 100++ sequential chemical reactions the intensities (sum of successful incorporations) and signal-to-noise (ratio of correct incorp to all three incorrect) would drop accordingly.
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