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yxl · 04-22-2011, 08:07 PM

I have several lanes of sequences which I have merged into one BAM file. I then processed this merged BAM file through GATK: recal, dedup, etc. I want create a pileup from the merged BAM file using GenomeAnalysisTK.jar -T Pileup . Is there a way to perform this pileup with the lanes separated? When I run Pileup on the merged BAM file, output exists, but it is unclear from which lane the output is generated.

04-22-2011, 08:07 PM	#1
yxl Junior Member Location: St. Louis Join Date: Feb 2011 Posts: 4	GATK pileup with merged BAM files I have several lanes of sequences which I have merged into one BAM file. I then processed this merged BAM file through GATK: recal, dedup, etc. I want create a pileup from the merged BAM file using GenomeAnalysisTK.jar -T Pileup . Is there a way to perform this pileup with the lanes separated? When I run Pileup on the merged BAM file, output exists, but it is unclear from which lane the output is generated.