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jjohnson · 01-05-2012, 10:35 AM

Hi Georgio,

I would check out CLCBio (or maybe another vendor - but we have used CLCBio for this). They have a great miRNA analysis pipeline. First you need to detect and trim the adapter, then cluster, and you can also annotate against know miRNAs (such as mIRBase, ncRNA.org, etc) using the software. Its free to try for 30 days, and if you like it, it usually pays for itself in short order, especially if you are doing other NGS stuff.

Let me know how it goes.

01-05-2012, 10:35 AM	#2
jjohnson Member Location: Washington DC Metro Area Join Date: Aug 2009 Posts: 20	Hi Georgio, I would check out CLCBio (or maybe another vendor - but we have used CLCBio for this). They have a great miRNA analysis pipeline. First you need to detect and trim the adapter, then cluster, and you can also annotate against know miRNAs (such as mIRBase, ncRNA.org, etc) using the software. Its free to try for 30 days, and if you like it, it usually pays for itself in short order, especially if you are doing other NGS stuff. Let me know how it goes. __________________ Justin H. Johnson \| Twitter: @BioInfo \| LinkedIn: http://bit.ly/LIJHJ \| EdgeBio