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Old 06-21-2012, 11:54 AM   #1
Frazzled
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Location: LA

Join Date: Jun 2012
Posts: 2
Default Questions about ERCC RNA Spike-In Control

Hi everyone

We're hoping to start an RNASeq run on the Illumina HiSeq SQ with 50 bases paired-end sequencing in the next few weeks. I recently came across the ERCC Spike-In Control, which lets you add known amounts of mRNA to your sample. I'm interested in using the ExFold mixes, which are two mixes with different amounts of the same mRNAs.

It looks like it could be really useful! But I want to hear experiences of people who have used it, any caveats when combining them into RNA sample in making the cDNA library and how well this control performs?

Any advice will be greatly appreciated

Thanks in advance,

Frazzled
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