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Old 04-29-2016, 05:55 AM   #1
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Location: Brazil

Join Date: Apr 2016
Posts: 2
Default Transforming stranded RNA-Seq libraries in unstranded

I want to detect differential gene expression by comparing two different RNA-Seq libraries, however one of them is stranded and the other one is unstranded. I know that unstranded libraries overrate the expression of RNAs that have a anti-sense transcript.

In order to eliminate this bias, can I align the stranded library in STAR as if it were a unstranded library? Will it eliminate the bias, or there is another factor that introduces a bias that prevents me from comparing these two libraries?

Thank you!
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