View Single Post
Old 04-29-2016, 08:47 AM   #2
Senior Member
Location: Bay Area

Join Date: Jun 2012
Posts: 114

I would assume that the comparison would be mostly valid as long as you both align and quantify the stranded library as though it were unstranded. Assuming the stranded library was done with the dUTP method, it amplifies the same fragments as would be present in an unstranded library, just uniformly only amplifying one strand or the other. There still might be some differences if different kits, most likely, or polymerases were used in library construction. If it wasn't done w/ dUTP, the method was probably different enough that it's hard to make the comparison.
cmbetts is offline   Reply With Quote