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Old 10-08-2019, 08:26 PM   #1
wei210
Junior Member
 
Location: Texas

Join Date: Jul 2019
Posts: 4
Default Kapa lib quant question

Hello. I am new here. I have a naive question. When we make series dilution for kapa lib quant.
1) Will it make big difference if dilute the lib using H2O vs 10mM tris PH8.0 w 0.05% tween-20 ?
2) will kapa conc typically higher than the Qubit conc (lib w Illumina p5/p7 sequence primer) ?
3) vetex to mix should be better than Pipet up/down. In the case using Pipet up/down. How many times up/down should be sufficient?
Thank you all so much
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