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Old 03-31-2012, 12:56 PM   #1
Ace5858
Junior Member
 
Location: New York

Join Date: Mar 2012
Posts: 8
Question Ladder-like bioanalyzer trace on supposed 150bp DNA sample library

I recently started working on very old hyde tissue, extracting DNA, running a HS Bioanalyzer chip. My results showed that the samples were heavily degraded down to 30bp to 50bp fragments. When I made the libraries, theoretically I should see 130bp to 150bp fragments with the adapters attached. I did a PCR amplification to enrich the libraries. I didn't size select because there was no larger pieces than 50bp in the original bioA run.

However, when I ran the second HS bioA chip with these libraries, I got this strange ladder-like read on my samples with extremely high basepairs.

Has anyone else experienced this same strange result?

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