Hi guys,
I made two small RNA libraries by using Truseq kit. I am looking for any kind of small RNA to identify any kind of virus in these two populations. After gel purification, I run libraries on chips from Bio-Rad (Experion). It seems that I have those expected sizes (147, 149, 154) but I also have 138 and 140 bp peaks (Attached). The last run is just blank! I was wondering if they are adapter dimers. I am still confused with 7 bp shifting of libraries on gel and bioanalyzer which illumina talks about that. Do you think it is better to make new libraries?
Thanks,
I made two small RNA libraries by using Truseq kit. I am looking for any kind of small RNA to identify any kind of virus in these two populations. After gel purification, I run libraries on chips from Bio-Rad (Experion). It seems that I have those expected sizes (147, 149, 154) but I also have 138 and 140 bp peaks (Attached). The last run is just blank! I was wondering if they are adapter dimers. I am still confused with 7 bp shifting of libraries on gel and bioanalyzer which illumina talks about that. Do you think it is better to make new libraries?
Thanks,
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