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Old 09-17-2013, 12:30 PM   #1
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Location: Vancouver

Join Date: Sep 2013
Posts: 3
Default Best method to prepare 100-200 bp DNA sample (Micrococcal Nuclease Digested)

Hi Everyone,

We're trying to map the nucleosome occupancy of a yeast. At the moment, I have micrococcal nuclease digested DNA fragments. These are around 100-200 bp long and I was wondering what kit or preparation would be best to prepare them for sequencing on the Illumina HiSeq system. Our lab has tried the Nextera XT kit, however this did not work because of the input DNA was less than 300 bp long. I was wondering if anyone knows anything about the TruSeq Nano DNA sample preparation kit or the TruSeq ChIP sample preparation kit and whether or not they would work for small fragmented DNA such as this?

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