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Old 10-27-2013, 12:15 PM   #2
Chipper
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Location: Sweden

Join Date: Mar 2008
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There will be some sequence-specific bias in the amplification but usually one compares the same sequences (genes) under different conditions, and with fragmented samples which makes it less of a problem. It is possible to use molecular identifiers (random sequences) in the adaptors to account for PCR bias (google qRNA-seq or "Counting absolute number of molecules using unique molecular identifiers" for more info).
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