View Single Post
Old 10-31-2013, 12:11 PM   #4
benjamir
Junior Member
 
Location: Houston, TX

Join Date: Nov 2012
Posts: 4
Default

Hi Mlacencio,
The Illumina TruSeq RNA-seq protocol calls for 15 cycle (total RNA 0.1 ug - 4 ug optimized).
A recent single-cell method, Quartz-Seq, performs 21 cycles of whole transcriptome amplification PCR prior to 10 - 12 cycles of library PCR.
benjamir is offline   Reply With Quote