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Old 11-08-2013, 04:49 AM   #1
MurielGB
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Location: Montpellier, France

Join Date: Oct 2013
Posts: 51
Default How to correctly choose Read Groups ?

Hello !

I need to add read groups to my BAM files before merging and variant calling with GATK.
Do you have any advice ?

I will use AddOrReplaceReadGroups.jar (picard) and plan to fill in the following tags : ID, library (LB), sequencing platform=Illumina (PL), and sample (SM).
Am I missing another important tag ?

Also, if I understand well, the ID should be unique to a sample, lane and sequencing experiment ?

Thanks,

Muriel
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