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Old 05-14-2014, 11:25 AM   #4
spf2
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Location: Vancouver

Join Date: Sep 2013
Posts: 3
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I used NEB's NEBNext® Ultra™ DNA Library Prep Kit for Illumina to generate nucleosome libraries for the yeast. They looked great on the bio analyzer/qubit, but over clustered on the miseq resulting in unindexed data with all of it being undetermined. I was wondering if you have any suggestions for optimizing the libraries for Illumina or any idea what may have went wrong?
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