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Old 06-04-2014, 06:10 AM   #13
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Quote:
Originally Posted by nucacidhunter View Post


I have seen this many times and have heard from others about it as well. But I never knew that Illumina explains the observation similar to what I independently came up with.
You say you have "seen it". This implies you have observed something. But SAV doesn't actually depict a cluster any differently that has been recognized by RTA from one that hasn't. Nor does SAV offer any way to verify the hypothesis that short amplicons produce brighter or more robust clusters than long amplicons.

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Phillip
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