View Single Post
Old 02-05-2015, 03:58 PM   #2
ScottC
Senior Member
 
Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246
Default

... or, has anyone out there used regular Taq to produce libraries? I guess that would answer the question about whether it will work. I think I recall someone in a thread about 16S survey sequencing saying that they generate their amplicons using regular Taq, but I don't know what they were using for their secondary PCR step.

Cheers,

Scott.
ScottC is offline   Reply With Quote