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Old 02-05-2015, 03:58 PM   #2
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Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246

... or, has anyone out there used regular Taq to produce libraries? I guess that would answer the question about whether it will work. I think I recall someone in a thread about 16S survey sequencing saying that they generate their amplicons using regular Taq, but I don't know what they were using for their secondary PCR step.


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