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Old 09-14-2017, 10:53 PM   #4
Genetic Librarian
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Join Date: May 2017
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We always amplified the mtGenome in two long range PCRs, so we were never limited in material.
At the end, our final libraries were quite large (fragment size) and output was good, so I guess it might work well with 0,5 ng as well.
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