View Single Post
Old 08-05-2011, 06:23 AM   #4
Robby
Member
 
Location: Germany

Join Date: Mar 2011
Posts: 68
Default

I think Picard is closer to the truth. If two reads have the same sequence, it is still possible, that it isn't a PCR-duplicate. If the reverse reads of two identical forward reads are different, it is probably no PCR-duplicate. But if the position and orientation of forward and reverse read are identical it is likely a PCR-duplicate. But of course, even the percentage calculated by Picard is overestimated.
Robby is offline   Reply With Quote