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Old 08-05-2011, 06:39 AM   #5
Location: paris

Join Date: Oct 2009
Posts: 86

Thanks for your explain.
Here you said Picard is overestimated. Does it mean Picard always give an overestimate duplication level?
Because Picard take bam/sam as input, it means that if I want to estimate the duplication level in my sequence. I must map the reads firstly. Is it possible take the fastq file to estimate the duplication level? Fastqc take the fastq files, but it seems not very correctly.
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