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Old 10-15-2012, 09:10 PM   #9
arkal
advancing one byte at a time!
 
Location: Bangalore, India

Join Date: Jun 2011
Posts: 56
Question

Quote:
Originally Posted by Robby View Post
I think Picard is closer to the truth. If two reads have the same sequence, it is still possible, that it isn't a PCR-duplicate. If the reverse reads of two identical forward reads are different, it is probably no PCR-duplicate. But if the position and orientation of forward and reverse read are identical it is likely a PCR-duplicate. But of course, even the percentage calculated by Picard is overestimated.
A trivial question: When you say overestimated, do u mean the duplication in his sample is LESS THAN 13% or MORE THAN 13%.
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