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Old 03-06-2013, 04:48 PM   #1
kerhard
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Location: Oakland

Join Date: Feb 2011
Posts: 27
Default Smaller than expected Bioanalyzer peak for size-selected RNAs?

Hello all,

I just got some strange Bioanalyzer results from their small RNA chip, was wondering if anyone had experienced anything similar. My intention is to make small RNA libraries from these samples, so I wanted to get a good idea of the input concentrations.

The RNA samples were size-selected from an 18% denaturing polyacrylamide gel - I cut out the region between ~25-30 bp as far as my size marker indicated as can be seen in the attached image - but the results from the Bioanalyzer indicated that the vast majority of my samples are 20 bp in size. Huh?

Is this just an indication of degradation of my samples? I would think that if this were the case, there would be a lot of smaller fragments present in the Bioanalyzer trace as well.

Any ideas on this would be greatly appreciated.

Karl
Attached Images
File Type: png Bioanalyzer_peaks.png (58.3 KB, 34 views)
File Type: png CUT_2s_zoom_cropped.png (28.5 KB, 12 views)
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