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Old 03-07-2013, 08:43 AM   #3
kerhard
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Location: Oakland

Join Date: Feb 2011
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Quote:
Originally Posted by kwaraska View Post
I agree with your assesment that there would be more on the Bioanalyzer if it was degraded.

Bioanalyzer in our hands can shift sizes by a few up to 10 bp, but normally that isn't a shift you would see.

I wouldn't worry too much. It is still the population you want regardless of the exact size, right?
Unfortunately, size is the main determination of my population of interest. If I were to make a library from this material, it would likely be strongly biased for miRNAs, which is not what I am interested in.

A shift of 5 bp in the Bioanalyzer run would certainly explain this result. I guess it's a matter of whether I believe the Bioanalyzer results or whether I believe the resolution of the gel.
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