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Old 06-12-2018, 07:24 PM   #295
GenoMax
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Location: East Coast USA

Join Date: Feb 2008
Posts: 6,800
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@horvathdp: You can provide NEBnext primers in a separate file as multi-fasta sequence. Then use that file with bbduk.sh. Also with paired-end reads use options "tpe tbo" to get residual bases at end of reads.
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