Hi there,
I'm new here and to NGS and hopefully you guys can help me
Has anyone NGS library preps on crosslinked chromatin? I am having some issues with the ligation of the Illumina adapters to my chromatin, any suggestions?
I am currently using the NEB T4 ligase, a 1:10 dilution of the adapters and between 1-10 ug of chromatin. Similar to the HiC method I digest my chromatin and fill in the overhangs using Klenow (exo).
Thanks!
I'm new here and to NGS and hopefully you guys can help me
Has anyone NGS library preps on crosslinked chromatin? I am having some issues with the ligation of the Illumina adapters to my chromatin, any suggestions?
I am currently using the NEB T4 ligase, a 1:10 dilution of the adapters and between 1-10 ug of chromatin. Similar to the HiC method I digest my chromatin and fill in the overhangs using Klenow (exo).
Thanks!