Hi,
I want to extract the "trace data" from the ab1 file, without calling bases. the traces from my experiment will look like this. With peaks scattered randomly throughout the trace. (It is not a normal sequencing read)
__________________|_______|______________________________
_______|____________________________________________|____
So I want the raw output from each channel, as an array.
Then I want to analyse it with Perl.
Has anyone got any ideas.
If you are interested, The experiment is RSCA, Reference Strand Conformational Analysis.
Thank you in advance.
I want to extract the "trace data" from the ab1 file, without calling bases. the traces from my experiment will look like this. With peaks scattered randomly throughout the trace. (It is not a normal sequencing read)
__________________|_______|______________________________
_______|____________________________________________|____
So I want the raw output from each channel, as an array.
Then I want to analyse it with Perl.
Has anyone got any ideas.
If you are interested, The experiment is RSCA, Reference Strand Conformational Analysis.
Thank you in advance.
Comment