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Old 11-30-2020, 09:45 AM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 453

No remodeling needed.
All Nextera libraries are PCR amplified to be sequenceable. After the PCR amplification they are wonderfully double-stranded throughout and should be blunt-ended (if a high-fidelity polymerase was used).

BTW, Truseq-style libraries have Y-adapters; Nextera/transposase libraries work differently.
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