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Old 09-26-2013, 08:37 PM   #3
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Location: Australia

Join Date: Jul 2013
Posts: 119

Originally Posted by Kennels View Post
No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.
thx~~ it is much clear to me now

Last edited by arkilis; 09-26-2013 at 09:24 PM.
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