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Old 01-09-2016, 03:08 PM   #2
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,076

Part that "it doesn't match any of the sequence from the input region that I used in mpileup' is worrisome. Are you sure about that?

If this was a stranded data you may want to check all three forward frames (or all 6 if not stranded).
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