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Old 05-13-2019, 05:29 AM   #1
Junior Member
Location: Sweden

Join Date: Aug 2018
Posts: 3
Default PCR amplicon normalization

Hi all.

I am looking for robust ways of normalizing concentrations of PCR amplicons.
I've previosuly done this by concentration measurement using either a nanodrop/qubit or from band intensity from gel electrophoresis.

I'm now looking into using magnetig beads (SPRI) insetad. The standard beads (like AMPure XP) are in excess when doing the regular PCR clean-up protocols, but thought that perhaps one could dilute them somehow in order to get a pre-determined output concentrations. From this one could also use home-made beads in order to reduce cost.

Does anybody have any experience in doing these sort of things?

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