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Old 05-14-2019, 05:21 PM   #4
Location: Brazil

Join Date: Aug 2015
Posts: 92

I'm sorry, did I misunderstand you? I thought you were considering libraries, and then what I meant is that qPCR with adaptor-specific primers is a better option than equalizing with SPRI beads. The concentration you get from qPCR is fairly accurate, and you can mix your amplicons in equimolar amount with good success. With SPRI beads we noticed that the amounts often weren't much equimolar.
If you mean regular PCR amplicons, I never trusted spectrophotometry below 20 ng/ul. I also used to quantify by gel, but currently I use spectrofluorimetry (a Qubit specifically, it works fine but I'm sure other brands work just as well).
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