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Old 10-23-2019, 05:03 AM   #4
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,076

Smaller inserts do tend to cluster more efficiently on flowcells. Can you tell us what the cluster density was? You may need to deliberately underload the run to keep cluster density in check and/or add more phiX.

It would be useful to have Illumina tech support look at the run metrics remotely and see what they say.
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