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Old 10-23-2019, 04:01 PM   #6
josh kinman
Location: Austin

Join Date: Apr 2014
Posts: 69

It's likely that you are just sequencing off of the end of the library, since your read length is longer than most of your inserts with adapter on the end.

Why use 300 cycles when you largest library size is 340bp, which means your largest insert size is at most ~220bp?

More PhiX will help and a 2X150 run would probably produce better results, but you'll still be sequencing off the end of any library that's shorter than 200bp so you might just want to use a 2X75.
Josh Kinman
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