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Old 10-24-2019, 02:10 AM   #7
Junior Member
Location: Michigan

Join Date: Aug 2012
Posts: 6

Honestly using the 300 cycles was a learning curve. Previously only needed 50 cycles kits so was initially told you put down the number of cycles of the kit in the sample sheet. The idea of sequencing through the entire insert didn't even occur to me. I hadn't quite pieced together until after the run failed that the you don't read through the entire library, only the insert (Still consider myself a newbie here). That being said we were able to recover some of the data from the failed run since it read straight through the index and our bioinformatician could pull out that information to index the sequences.
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