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Old 05-14-2012, 11:45 AM   #7
Location: Florida

Join Date: Nov 2011
Posts: 22

MinION does sound amazing, but I don't think I can wait that patiently

What about the methods described by Lindsay, Bonfield, and Hurles (2005) "Shotgun haplotyping: a novel method for surveying allelic sequence variation" Nuc Acids Res 33(18)

They suggest that haplotypes for long PCR products can be phased using paired-end reads and high coverage. If so, PacBio would even side-step the need for paired-end reads, as polymorphisms should show up within their ~3 kb reads and allow for assembly of each allele. Anyone more familiar with error rates and such think this is plausible?
HeidiJTP is offline   Reply With Quote