I am new to pyrosequencing. We got some 16s rRNA data from sequencing center. It's fasta format file. they give me two set of the data:
prepared 2 sets of files (zips attached);
1) reads for each amplicon starting from the FLXA side (> 200 bp)
2) reads for each amplicon starting from the FLXB side (> 200 bp)
What's does that mean?
We are using that to estimate the biodiversity in the sample. which data set I should use?
They didn't give me quality file either. Is the quality file very important in the quality control step of the data process?
prepared 2 sets of files (zips attached);
1) reads for each amplicon starting from the FLXA side (> 200 bp)
2) reads for each amplicon starting from the FLXB side (> 200 bp)
What's does that mean?
We are using that to estimate the biodiversity in the sample. which data set I should use?
They didn't give me quality file either. Is the quality file very important in the quality control step of the data process?
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