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Old 12-08-2017, 04:28 AM   #9
Genetic Librarian
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Join Date: May 2017
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Quote:
Originally Posted by clseq2017 View Post
and how do I lower the loading concentration?
During the denature and dilute process you dilute your library to the final loading concentration with the provided buffer based on your final Qubit quantification of the library pool. So, instead of diluting it to e.g. 10 pM loading concentration, try 6 pM next time.
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