Hello.
We are aiming to perform RNA sequencing on stem cells. This means that it will be hard to get enough of starting material, somewhere between 50-100ng total RNA per replicate (Running for biological triplicates) is realistic to assume.
We would like to investigate differently expressed genes and non coding RNAs, this means that rRNA depletion is of interest on a HiSeq
I have been in contact with Illumina and they tell me that they guarantee that their Truseq works perfectly using 100ng input material but many of out samples will be around 50ng.
My question is which kit to use for this kind of "low but not ultra low" input material?
Thank you for helping.
Kind regards,
Anders
We are aiming to perform RNA sequencing on stem cells. This means that it will be hard to get enough of starting material, somewhere between 50-100ng total RNA per replicate (Running for biological triplicates) is realistic to assume.
We would like to investigate differently expressed genes and non coding RNAs, this means that rRNA depletion is of interest on a HiSeq
I have been in contact with Illumina and they tell me that they guarantee that their Truseq works perfectly using 100ng input material but many of out samples will be around 50ng.
My question is which kit to use for this kind of "low but not ultra low" input material?
Thank you for helping.
Kind regards,
Anders
Comment