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Old 04-01-2010, 07:44 AM   #20
sparks
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Location: Kuala Lumpur, Malaysia

Join Date: Mar 2008
Posts: 126
Default Colour Space

Hi Sebastien,
My apologies re name mix up. We have two lanes of 50bp PE from a bacteria to assemble in next few weeks so well give Ray a try. I'm thinking assembly in colour space isn't much different to that in nucleotide space but after CS assembly we need to convert back to Nucleotide. This could mean remembering first colour of all the reads and their positions in the contigs as first colour and primer base gives a reference for conversion. Are you doing this?
Thanks for giving us Ray. We'll let you know how it goes.
Colin

Quote:
Originally Posted by seb567 View Post
Dear sparks,

You are right. I changed +1 to +2 to skip the first color too.

p.s.: I (Sébastien Boisvert) developed Ray.
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