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Old 05-14-2014, 11:01 PM   #1
Junior Member
Location: japan

Join Date: May 2014
Posts: 3
Default End repair of TruSeq RNA and TruSeq mRNA stranded

Hi there,

I have a question about the Library constranstion of TruSeq RNA and stranded mRNA prep kit.
In the TruSeq RNA, reverse transcripted second strand cDNA is subject to End repair processing, but in the TruSeq stranded mRNA, that is not subject to End repair.
Why the second strand cDNA that is synthesized by the TruSeq stranded mRNA kit is not required the end repair processing?

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