SEQanswers - View Single Post - Disagreement between bioanalyzer and agarose gel for PCR amplicon library

tomhooven · 07-30-2015, 04:45 PM

Hi Michael--
I was curious how this worked out for you. I recently prepared an amplicon library for TnSeq and had a similar discrepancy between my gel (the expected 190-bp band) and my bioanalyzer results (around 220 bp). I couldn't figure out why this happened.
-Tom

07-30-2015, 04:45 PM	#4
tomhooven Junior Member Location: New York City Join Date: Jul 2015 Posts: 1	Hi Michael-- I was curious how this worked out for you. I recently prepared an amplicon library for TnSeq and had a similar discrepancy between my gel (the expected 190-bp band) and my bioanalyzer results (around 220 bp). I couldn't figure out why this happened. -Tom