SEQanswers - View Single Post - Disagreement between bioanalyzer and agarose gel for PCR amplicon library

DNA_Dan · 08-03-2015, 10:28 AM

I think this has to do with the mobility of the y-forked adapters. Agarose is more difficult for migration because it's a solid phase, whereas the Bioanalyzer chip's gel matrix is much more fluid. I think the DNA 7500 chip gives the best representation of the "real" size.

08-03-2015, 10:28 AM	#5
DNA_Dan Member Location: Montana Join Date: Nov 2008 Posts: 21	I think this has to do with the mobility of the y-forked adapters. Agarose is more difficult for migration because it's a solid phase, whereas the Bioanalyzer chip's gel matrix is much more fluid. I think the DNA 7500 chip gives the best representation of the "real" size.