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Old 05-11-2017, 05:58 PM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 452

Hi Anjama,

I would use the Bioanalyzer molarity values generate for ''regions" with the boundaries set according to your preferences.

Adapter dimers can be removed by magnetic-bead cleanups which would be easier (if the dimers are not excessive).
Will you be doing single-end or paired-end sequencing?
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