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Old 05-12-2017, 05:42 PM   #3
anjama
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Location: Florida

Join Date: Mar 2016
Posts: 15
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Quote:
Originally Posted by luc View Post
Will you be doing single-end or paired-end sequencing?
For the species I'm working with it'll probably be single-end, but other people might do short paired-end reads where they overlap

I have some ligation products left over from our last titration attempt, and we have all the stuff necessary to do a gel extraction, so I'm going to give that a try tomorrow because it's cheap to do and we have no other use for these ligation products at this point.

I'm only superficially familiar with how magnetic-bead cleanups work, but have no experience with it. We do have access to the hardware necessary, and I think all we need is the beads themselves, so I'll look into that as well.
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