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Old 06-09-2017, 07:02 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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Some possible causes:

1- variation in pipetting and pipette being out of calibration
2- using low sample volume. For comparisons it is better to use minimum 3ul sample or the standard with two replicates
3- using unsuitable Qubit reagent and buffer, best practice would be using sample with concentration around 10 ng/ul with HS reagents
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