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Old 06-12-2017, 07:41 AM   #3
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

Remember quibit is a single point calibration (with blank-s1 0ng, s2 10ng). So the further you are from 10ng/ul the worse the quantification will be. Are you doing replicates with your picogreen? (you can do 25ul diluted dye, 25ul diluted DNA in 384 well plates to minimize costs and allow you to do replicates)

The nanodrop is going to be way off, don't worry about the numbers it comes up with.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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