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Old 07-02-2017, 09:56 PM   #2
Location: Honolulu, HI

Join Date: Jul 2015
Posts: 40

I might be stating the obvious here, but why not try 2.5M NaCl with 8% PEG? I can't explain why the other protocols seem to be working, but I really question whether it's necessary to reduce NaCl concentration so dramatically.

Also, stupid question: when you say "final mix," you mean with the sample (DNA) already included, correct?

Last edited by Carcharodon; 07-02-2017 at 10:02 PM.
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