SEQanswers - View Single Post - Optimizing magnetic bead mix for gDNA extraction

Carcharodon · 07-02-2017, 09:56 PM

I might be stating the obvious here, but why not try 2.5M NaCl with 8% PEG? I can't explain why the other protocols seem to be working, but I really question whether it's necessary to reduce NaCl concentration so dramatically.

Also, stupid question: when you say "final mix," you mean with the sample (DNA) already included, correct?

07-02-2017, 09:56 PM	#2
Carcharodon Member Location: Honolulu, HI Join Date: Jul 2015 Posts: 40	I might be stating the obvious here, but why not try 2.5M NaCl with 8% PEG? I can't explain why the other protocols seem to be working, but I really question whether it's necessary to reduce NaCl concentration so dramatically. Also, stupid question: when you say "final mix," you mean with the sample (DNA) already included, correct? Last edited by Carcharodon; 07-02-2017 at 10:02 PM.