SEQanswers - View Single Post - Optimizing magnetic bead mix for gDNA extraction

ATϟGC · 07-04-2017, 06:13 AM

I have always thought of the NaCl (or another source of Na+ like Sodium acetate) as a co-factor required for an ethanol, isopropanol or PEG-base nucleic acid precipitation. NaCl might precipitate nucleic acids to a degree but I do not think that it is nearly as efficient as alcohols of PEG.

07-04-2017, 06:13 AM	#3
ATϟGC Member Location: Canada Join Date: Jun 2013 Posts: 56	I have always thought of the NaCl (or another source of Na+ like Sodium acetate) as a co-factor required for an ethanol, isopropanol or PEG-base nucleic acid precipitation. NaCl might precipitate nucleic acids to a degree but I do not think that it is nearly as efficient as alcohols of PEG.