Quote:
Originally Posted by ATϟGC
I have always thought of the NaCl (or another source of Na+ like Sodium acetate) as a co-factor required for an ethanol, isopropanol or PEG-base nucleic acid precipitation. NaCl might precipitate nucleic acids to a degree but I do not think that it is nearly as efficient as alcohols of PEG.
|
I think that the sodium ions, in this case, may be somehow facilitating the binding of DNA to the carboxylated surface of the beads rather than playing a large role in the actual precipitation.