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Old 01-05-2018, 01:40 PM   #3
omans
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Location: New York

Join Date: Jan 2018
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Primary lymphocytes have a significantly lower level of mRNA than cultured cells.

I've found that scRNA-seq is very sensitive to sample handling. Are you using fresh or frozen T cells? How long is your sort?

Also, how many PCR cycles did you perform for each of these samples? For other lymphocyte types, I've needed to exceed 21 cycles to see a peak.
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