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Old 01-05-2018, 02:07 PM   #4
Junior Member
Location: CT

Join Date: Jan 2018
Posts: 8

I have tried increasing the PCR cycles up to 23 but still got this double-bump pattern.
The samples were frozen, I sort directly into lysis buffer (max 5min per plate), directly spin the plate down and then freeze on dry-ice and transfer to -80C until processing. For processing, I start out with bringing the RNA beads to room temp, RNAzap the work area, let the plates thaw on ice for 10min and then start the protocol.
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